However, the Premier League club ended their pursuit of the Croatia worldwide on Deadline Day.
However, the arrival of Mandzukic also means that Al Duhail's young forwards Almoez Ali and Mohanad Ali will fall further down in the pecking order when it comes to place in the Red Knights' starting 11.
The 33-year-old will team up with the current QSL table leaders, coached by Portuguese Rui Faria in the coming days.
The former Croatia worldwide, who did not make a single appearance under new Juventus manager Maurizio Sarri this season, joins former Juve teammate Medhi Benatia at Al Duhail.
Despite being heavily linked with the Red Devils once again, the 33-year-old has now made a decision to put pen-to-paper on a deal with the Qatari club. Internationally, he scored 33 goals in 89 appearances for Croatia, making the final of the 2018 World Cup won by France.
Mandzukic, a World Cup runner-up with Croatia last summer, has been frozen out at Juventus as he neared the end of a four-year deal, which he signed when joining from Atletico Madrid in 2015.
He retired from worldwide football after the World Cup in 2018 after scoring 33 goals for his national team.